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KMID : 0616120200510020096
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Korean Journal of Pharmacognosy
2020 Volume.51 No. 2 p.96 ~ p.99
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Isolation of the Constituents from Clinopodium chinense var. shibetchense and Inhibition Activity on Cancer Cell Growth and Nitric Oxide Production
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Kim Dong-Hwan
Lee Sang-Kook
Park Kyoung-Sik
Park Hee-Juhn
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Abstract
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This study was performed to find anti-inflammatory or antitumor compounds from the polar fraction obtained from the extract of Clinopodium chinense var. shibetchense (H. Lev) Koidz (Labiatae). Chromatography of the BuOH fraction yielded two flavonoid glycosides (compounds 1 and 2) and two saponins (compounds 3 and 4). On the basis of spectroscopic data, compounds 1 and 2 were identified to be ponciretin 7-O-a-L-rhamnopyranosyl-(1®6)-b-D-glucopyranoside (neoponcirin) and naringenin 7-O-a-L-rhamnopyranosyl-(1®6)-b-D-glucopyranoside (isonaringin). Compounds 3 and 4 were identified to be 3-O-{β-D-glucopyranosyl-(1®2)-[β-D-glucopyranosyl-(1®3)]-β-D-fucopyranosyl}-saikogenin F (buddlejasaponin IV) and 3-O-{β-D-glucopyranosyl-(1®2)-[β-D-glucopyranosyl-(1®3)]-β-D-fucopyranosyl}-21β-hydroxysaikogenin F (clinoposaponin XV). In addition, ursolic acid (5) was isolated and identified from the CHCl3 fraction. Inducible nitric oxide synthase (iNOS) assay and sulforhodamine B (SRB) assay were performed to lead a potential anti-inflammatory or anti-tumor compounds from C. chinense var. shibetchense. Of the four compounds (1 ? 4), compound 3 considerably inhibited cancer cell growth and NO production (IC50s, 5.59 μM in iNOS assay and 6.62 - 14.88 μM in SRB assay).
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KEYWORD
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Clinopodium chinense var, shibetchense, Labiatae, Flavonoid glycoside' Saponin' iNOS assay, SRB assay
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